Free Scientific Poster

Pure Synthetic oDNA for mRNA Therapies

Faster, Higher-Quality, More Efficient mRNA Development

What happens when you replace linearized plasmid DNA with a synthetic, cell-free template for in vitro transcription? This poster puts the two head to head, from enzyme kinetics through a CEPI-funded vaccine program.

  • +30%mRNA yield vs plasmid DNA
  • 90%mRNA integrity without additional purification
  • −30%dsRNA reduction vs plasmid DNA
Scientific Poster
Revolutionizing mRNA Therapies
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What’s Included

The Data Behind Switching Your IVT Template

Plasmid DNA has been the default IVT template for years, and it brings bacterial fermentation, linearization, and long lead times with it. Artis oDNA is a synthetic, double-stranded linear DNA made in a fully cell-free enzymatic process. This poster shows how it performs as an mRNA template, with data from our own studies, more than 30 customer programs, and a vaccine program developed with Afrigen.

Head-to-Head IVT Results

oDNA vs linearized plasmid DNA on mRNA yield, integrity, dsRNA content, and in vivo functional response.

Engineered Adaptors

How adaptors designed around the T7 RNA polymerase structure raise Vmax and lower Km for faster transcription.

A CEPI-Funded Case Study

Afrigen’s Rift Valley Fever mRNA vaccine: higher IVT yield, matched integrity and dsRNA, and higher protein expression.

Feedback From 30+ Programs

Pairwise customer comparisons on IVT yield, dsRNA, and mRNA functionality across GOIs from 1 to 15 kb.

What’s Inside

From Template Design to a Working Vaccine

What oDNA Is and How Adaptors Work

A synthetic linear template containing only your gene of interest, with optional adaptors configured as closed, open, or partially open ends.

The Cell-Free Manufacturing Process

Two routes to gram-scale oDNA, from a synthetic or plasmid starting material, with in-process controls after each step.

Functional Evaluation as an IVT Template

Yield, integrity, and dsRNA against plasmid DNA, plus in vivo expression after LNP-mRNA injection in mice.

Customer Experience in the mRNA Field

How oDNA compared with plasmid DNA across more than 30 customer programs, with poly(A) tails from 40 to 135 bp.

Case Study: Rift Valley Fever Vaccine

Afrigen’s results on yield, dsRNA against its acceptance criteria, and Gn protein expression by flow cytometry.

The Added Value Over Plasmid DNA

Ready-to-use templates with no linearization, gram-scale output, shorter delivery times, and the impact on cost and quality.

Developed with Afrigen, DNA Script, Ribbon, and CIMA, University of Navarra. Case study funded by CEPI.

Working on an mRNA Program?
We can run your sequence as oDNA and show you the comparison against your current plasmid template on your own IVT process.
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